Molar Extinction Coefficient Determination: securing your protein assays
The molar extinction coefficient (ε) is an essential parameter for accurately quantifying the concentration of a protein or nucleic acid in solution using the Beer-Lambert law.
In the development of biomolecules (mAbs, recombinant proteins), an error in this coefficient leads to an incorrect assessment of yield and dosage, which can jeopardize stability studies and clinical trials.
FILAB laboratory offers a rigorous experimental determination of your molar extinction coefficient, moving beyond simple theoretical estimations based on amino acid sequences to account for the molecule’s tertiary structure and chemical environment.
Our Extinction Coefficient Analysis Services
FILAB helps you establish the exact correlation between absorbance and mass concentration:
Experimental (ε) determination: Direct measurement by coupling UV spectrophotometry with an absolute quantification method.
Linearity validation: Verification of absorbance response over a range of defined concentrations to ensure the reliability of your routine protocols.
Amino Acid Analysis (AAA): The gold standard method for precisely quantifying protein mass after total acid hydrolysis, allowing for the calculation of the real extinction coefficient.
Physicochemical characterization requirements (ICH Q6B).
Our other biopharmaceutical analysis services
Identification and quantification of PTMs
Analysis according to ICH 6B guideline
Analysis and localisation of disulfide bridges
Protein analysis using the following methods: BCA method, Lowry method, CBQCA method, Bradford method
Why choose FILAB ?
- Multidisciplinary expertise: a team of PhDs and engineers specializing in analytical chemistry and biochemistry.
- Dual chemistry / materials expertise
- ISO 17025 accreditation by COFRAC.
- 5,200 m² state-of-the-art analytical facility
- GMP environment